Composite

Part:BBa_K3734024:Design

Designed by: Xingjun Zhao   Group: iGEM21_CSU_CHINA   (2021-10-01)


miR21T-LUC-4XmiR21T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 166
    Illegal NgoMIV site found at 1510
    Illegal NgoMIV site found at 1531
    Illegal AgeI site found at 1234
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1416


Design Notes

We use 4XmiR21T at 3'UTR.When you use PCR to clone the fragment, you need to focus on how many times the miR21T repeats.


Source

CHREBP and miR21T are from human genome. LUC is from glowworm.

References